Preparation and Quality Control of 68Ga-Citrate for PET Applications

Document Type: Original Article

Authors

Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran

Abstract

 
Objective(s): In nuclear medicine studies, gallium-68 (68Ga) citrate has been recently known as a suitable infection agent in positron emission tomography (PET). In this study, by applying an in-house produced 68Ge/68Ga generator, a simple technique for the synthesis and quality control of 68Ga-citrate was introduced; followed by preliminary animal studies.
Methods: 68GaCl3 eluted from the generator was studied in terms of quality control factors including radiochemical purity (assessed by HPLC and RTLC), chemical purity (assessed by ICP-EOS), radionuclide purity (evaluated by HPGe), and breakthrough. 68Ga-citrate was prepared from eluted 68GaCl3 and sodium citrate under various reaction conditions. Stability of the complex was evaluated in human serum for 2 h at 370C, followed by biodistribution studies in rats for 120 min.
Results: 68Ga-citrate was prepared with acceptable radiochemical purity (>97 ITLC and >98% HPLC), specific activity (4-6 GBq/mM), chemical purity (Sn, FeConclusion: This study demonstrated the possible in-house preparation and quality control of 68Ga-citrate, using a commercially available 68Ge/68Ga generator for PET imaging throughout the country.
 

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Main Subjects


 

  1. Firestone RB, Shirley VS, Baglin CM, Zipkin J. Table of isotopes. 8th edition. New York: John Wiley and Sons; 1996. p. 1447.
  2. DDEP, Decay Data Evaluation Project Data. http:// www.nucleide.org/DDEP_WG/DDEPdata.htm (2008).
  3. Burleson RL, Johnson MC, Head H. Scintigraphic demonstration of experimental abscesses with intravenous 67Ga citrate and 67Ga labeled blood leukocytes. Ann Surg. 1973; 178(4):446-52.
  4. Jalilian AR, Novinrooz A, Motamedi-Sedeh F, Moradkhani S, Rajamand AA, Solati J. Evaluation of [67Ga]Citrate in The Detection of Various Microorganism Infections in Animal Models. Iran J Nucl Med. 2009;17(2):34-41.
  5. Nanni C, Errani C, Boriani L, Fantini L, Ambrosini V, Boschi S, et al. 68Ga-citrate PET/CT for evaluating patients with infections of the bone: preliminary results. J Nucl Med. 2010;51(12):1932-6.
  6. Kumar V, Boddeti DK, Evans SG, Angelides S. (68) Ga-Citrate-PET for diagnostic imaging of infection in rats and for intra-abdominal infection in a patient. Curr Radiopharm. 2012;5(1):71-5.
  7. Jensen SB, Nielsen KM, Mewis D, Kaufmann J. Fast and simple one-step preparation of 68Ga citrate for routine clinical PET. Nucl Med Commun. 2013; 34(8):806-12.
  8. Rizzello A, Di Pierro D, Lodi F, Trespidi S, Cicoria G, Pancaldi D, et al. Synthesis and quality control of 68Ga citrate for routine clinical PET. Nucl Med Commun. 2009;30(7):542-5.
  9. Vorster M, Mokaleng B, Sathekge MM, Ebenhan T. A modified technique for efficient radiolabeling of 68Ga-citrate from a SnO2-based 68Ge/68Ga generator for better infection imaging. Hell J Nucl Med. 2013;16(3):193-8.
  10. Kumar V, Boddeti DK, Evans SG, Angelides S. (68) Ga-Citrate-PET for diagnostic imaging of infection in rats and for intra-abdominal infection in a patient. Current Radiopharmaceut. 2011; 5(1):71-5.
  11. Silvola JM, Laitinen I, Sipilä HJ, Laine VJ, Leppänen P, Ylä-Herttuala S, et al. Uptake of gallium-68 in atherosclerotic plaques in LDLR-/- ApoB100/100 mice. EJNMMI Res. 2011;1(1):14.
  12. Jalilian AR, Bineshmarvasti M, Sardari S. Application of radioisotopes in Inflammation. Curr Med Chem. 2006;13(8):959-65.
  13. Fazaeli Y, Jalilian AR, Ardaneh K, Rahiminejad A, Bolourinovin F, Moradkhani S, et al. Development of a 68Ga-fluorinated porphyrin complex as a possible PET imaging agent. Nucl Med Mol Imaging. 2012; 46(1):20-6.
  14. Jalilian AR, Rowshanfarzad P, Sabet M, Novinrooz A, Raisali G. Preparation of [66Ga]Bleomycin Complex as a Possible PET Radiopharmaceutical. J Radioanal Nucl Chem. 2005; 264: 617-21.
  15. Kamada M. Distribution of 67Ga-citrate in tumor tissues and various organs. Radioisotopes (Tokyo). 1978; 27(7):390-396.
  16. Gallium (68Ga) endotreotide injection. Pharmeuropa. 2011; 23(2):310-3.
  17. Jalilian AR, Yousefnia H, Zolghadri S, Khoshdel MR, Bolourinovin F, Rahiminejad A. Development of radiogallium–ethylenecysteamine cysteine complex as a possible renal imaging agent. J Radioanal Nucl Chem. 2010; 284:49–54