TY - JOUR ID - 12903 TI - Evaluation of 99m Tc-MccJ25 peptide analog in mice bearing B16F10 melanoma tumor as a diagnostic radiotracer JO - Asia Oceania Journal of Nuclear Medicine and Biology JA - AOJNMB LA - en SN - 2322-5718 AU - Mazaheri Tehrani, Maryam AU - Erfani, Mostafa AU - Amirmozafari, Nour AU - Nejadsattari, Taher AD - Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran. AD - Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran AD - Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Y1 - 2019 PY - 2019 VL - 7 IS - 2 SP - 172 EP - 180 KW - 99m Tc-MccJ25 KW - Radiotracer KW - B16F10 KW - Tumor DO - 10.22038/aojnmb.2019.37712.1251 N2 - Objective(s): Despite recent advances in treatment modalities, cancer remains a major source of morbidity and mortality throughout the world. Currently, the development of sensitive and specific molecular imaging probes for early diagnosis of cancer is still a problematic challenge. Previous studies have been shown that some of the antimicrobial peptides (AMPs) exhibit a broad spectrum of cytotoxic activity against cancerous cells in addition to their antimicrobial activities. MicrocinJ25 (MccJ25) is an antimicrobial peptide that is produced by Escherichia coli (E. coli) strain. The aim of this study was to investigate the potential of a new peptide radiopharmaceutical derived from MccJ25 for diagnosis of melanoma tumor bearing C57BL/6 mice. Methods: A 14 amino acid analog of MccJ25 was labeled with technetium-99m (99mTc) through hydrazinonicotinamide (HYNIC) chelator and tricine as coligand. In vivo tumor uptake and tissue distribution were evaluated. The in vivo biodistribution studies were determined in C57BL/6 mice bearing B16F10 tumor. Results: The amount of non-peptide related 99mTc-impurities that measured by thin layer chromatography (TLC) did not exceed 5% of the total radioactivity. The in vitro binding to B16F10 cells was 30.73 ± 0.9% after 1 h incubation at 37°C, and saturation binding experiments showed good affinity for radio-complex (Kd=47.98±6.25 nM). The melanoma tumor was clearly visible up 1 h post-injection by gamma camera imaging. Conclusion: The results showed that 99mTc-labeld peptide could be a promising candidate as a targeting radiopharmaceutical for melanoma tumor imaging in mice. UR - https://aojnmb.mums.ac.ir/article_12903.html L1 - https://aojnmb.mums.ac.ir/article_12903_a7dd397681ab9ac8fd1065dbef23723e.pdf ER -