TY - JOUR ID - 19478 TI - Evaluation of [99mTc][Tc-HYNIC/EDDA]-Tyr as a target for metabolic tumor imaging in B16F10 melanoma tumor JO - Asia Oceania Journal of Nuclear Medicine and Biology JA - AOJNMB LA - en SN - 2322-5718 AU - Yaghoubi Mogadam, Hemat AU - Erfani, Mostafa AU - Nikpassand, Mohammad AU - Mokhtary, Masoud AD - Department of chemistry, Rasht Branch, Islamic Azad University, Rasht, Iran AD - Radiation Applications Research School, Nuclear Science and Technology Research Institute, Tehran, Iran Y1 - 2022 PY - 2022 VL - 10 IS - 2 SP - 100 EP - 108 KW - Protein metabolism KW - Labeled amino acid KW - Imaging agent KW - Melanoma DO - 10.22038/aojnmb.2021.60334.1420 N2 - Objective(s): Clinical interest in metabolic imaging of cancer has been growing in recent years. The increase in protein metabolism of cancer cells is interesting target for metabolic tumor imaging, for which radiolabeled amino acids can be applied. The aim of this study was to evaluate a newly developed radiolabeled amino acid as an imaging protein metabolism in melanoma tumor.Methods: The radiolabeled tyrosine ([99mTc][Tc-HYNIC/EDDA]-Tyr) was prepared and its biological  properties was evaluated in B16F10 melanoma tumor. Moreover organs uptake and tumor accumulation were measured in mouse bearing B16F10 melanoma tumor.Results: Radiolabeled tyrosine was attached in B16F10 melanoma cells and showed the cell binding capacity of 13.82±0.73%. In animal study, the accumulation of radiolabeled tyrosine was observed in B16F10 melanoma tumor (2.15±0.09 %ID/g) after 30 min post injection, so that the uptake ratio of tumor to muscle was about 5.11. Through scintigraphy process the melanoma tumor clearly visualized in mice at 30 min post injection.Conclusion: These data suggest that the novel radiotracer ([99mTc][Tc-HYNIC/EDDA]-Tyr) as an protein metabolism imaging agent, is able to transfer into melanoma cells and show great expectation for the clinical application in the imaging of melanoma tumors. UR - https://aojnmb.mums.ac.ir/article_19478.html L1 - https://aojnmb.mums.ac.ir/article_19478_ec1603588fc41c93a46edefa5588c784.pdf ER -